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Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
Published on: November 25, 2017
Altered protein mannosylation in developing cerebral cortex by streptomycin
M D Alperin1, J M Calandria, H Carminatti
1Instituto de Investigaciones Bioquímicas Fundación Campomar, FCEyN UBA, Buenos Aires, Argentina.
Neuroreport
|November 30, 2000
Summary
Streptomycin alters protein mannosylation in developing rat brain tissue, specifically impacting N-linked oligosaccharides. This finding suggests potential neurological toxicity from this antibiotic agent.
Area of Science:
- Biochemistry
- Neuroscience
- Developmental Biology
Background:
- Streptomycin is an antibiotic with potential neurotoxic effects.
- Postnatal brain development involves complex biochemical processes, including protein glycosylation.
Purpose of the Study:
- To investigate the biochemical impact of streptomycin on postnatal rat cerebral cortex development.
- To characterize changes in protein mannosylation and related pathways.
Main Methods:
- Utilized mini-tissue units from P5 rat cerebral cortex to preserve in situ topological relationships.
- Assessed mannosylation of asparagine-linked oligosaccharides, polypeptide synthesis, DNA synthesis, and nutrient uptake.
- Measured the synthesis of Dolichylpyrophosphate-linked oligosaccharides and oligosaccharyltransferase activity.
Main Results:
- Streptomycin exposure decreased mannosylation of asparagine-linked oligosaccharides.
- No significant changes were observed in polypeptide synthesis, DNA synthesis, or glucose/mannose metabolism.
- The synthesis of Dolichylpyrophosphate-GlcNAc2Man9Glc3 and oligosaccharyltransferase activity remained unaffected.
Conclusions:
- The observed reduction in protein mannosylation occurs downstream of G3 transfer to nascent polypeptides.
- Rat cerebral cortex mini-tissue units are a viable model for assessing the neurological toxicity of antibacterial agents like streptomycin.
