Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse beta(6) integrin sequence, pattern of expression, and role in kidney development.

Lois J Arend1, Ann M Smart2, Josie P Briggs2

  • 1Department of Pathology, University of Michigan, Ann Arbor, Michigan.

Journal of the American Society of Nephrology : JASN
|November 30, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methotrexate Crystals on Electron Microscopy of Kidney Biopsy for Acute Kidney Injury.

Kidney medicine·2024
Same author

Fast Processing of Electron Microscopic Specimen Preserved Ultrastructure of Glomeruli and Electron-Dense Deposits in Diagnostic Renal Biopsies: A Prospective and Retrospective Comparative Study.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2024
Same author

Clinicopathological characteristics of pediatric ANCA-associated glomerulonephritis.

Pediatric nephrology (Berlin, Germany)·2024
Same author

Double-negative T cells have a reparative role after experimental severe ischemic acute kidney injury.

American journal of physiology. Renal physiology·2024
Same author

Single Nucleus RNA Sequencing of Remnant Kidney Biopsies and Urine Cell RNA Sequencing Reveal Cell Specific Markers of Covid-19 Acute Kidney Injury.

bioRxiv : the preprint server for biology·2023
Same author

Ichthyosis in sarcoidosis: a rare skin manifestation of a systemic disease.

Lancet (London, England)·2023
Same journal

Variants to Functions to Therapeutic Strategies Toward Genomically Informed Care for Autosomal Dominant Polycystic Kidney Disease.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Personalizing Cardio-Kidney-Metabolic Therapy: Closer But Not There Yet.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Autosomal Dominant Tubulointerstitial Kidney Disease: My Kingdom for a Biomarker.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Beyond the Margin: Improving Noninferiority Trials of Kidney Transplant Immunosuppression.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Parathyroid Hormone Receptor 1 Facilitates Cyst Growth in Genetic Models of Autosomal Dominant Polycystic Kidney Disease.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Alanyl-tRNA Synthetase 1 and Cyst Growth in Autosomal Dominant Polycystic Kidney Disease.

Journal of the American Society of Nephrology : JASN·2026
See all related articles

Beta-6 integrin plays a crucial role in mouse kidney development. Its expression is high in developing kidneys and essential for ureteric bud branching and mesenchyme condensation.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Integrins are crucial for cell-cell and cell-extracellular matrix interactions during development.
  • Beta-6 integrin is expressed at higher levels in human fetal kidneys than adult kidneys, suggesting a role in nephron development.

Purpose of the Study:

  • To determine the cDNA sequence for mouse beta-6 integrin.
  • To characterize beta-6 integrin expression in the developing mouse kidney.

Main Methods:

  • Screening of embryonic mouse kidney cDNA libraries and sequencing.
  • Reverse transcription-polymerase chain reaction (RT-PCR) to analyze mRNA expression.
  • In vitro culture of metanephric explants with antisense oligonucleotides.

Related Experiment Videos

Main Results:

  • Mouse beta-6 integrin shares high nucleotide (82%) and amino acid (89.5%) identity with human beta-6 integrin.
  • Beta-6 integrin mRNA expression is low at early gestation, increases significantly by E14/E17, and is maintained into adulthood.
  • Expression is induced in metanephric explant cultures and found in various adult rat nephron segments.
  • Inhibition of beta-6 integrin in culture disrupts ureteric bud branching and mesenchyme condensation.

Conclusions:

  • Mouse and human beta-6 integrin sequences are highly homologous.
  • Beta-6 integrin exhibits distinct expression patterns in developing mouse kidneys compared to primates.
  • Beta-6 integrin is essential for normal mouse kidney development, particularly ureteric bud branching and mesenchyme condensation.