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NMR study on the interaction between MHC class I protein and its antigen peptide

M Nakagawa1, K Chiba-Kamoshida, K Udaka

  • 1National Institute of Bioscience and Human-Technology, AIST, 1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.

Biochemical and Biophysical Research Communications
|November 30, 2000
PubMed

A major histcompatibility complex (MHC) class I protein H-2K(b) was expressed in a large scale as a fusion protein with thioredoxin and hexahistidine at the N-terminus to analyze the interaction with the antigen peptide SIYRYYGL. NMR spectra of the peptide in the mixture solution with the protein showed very broad signals, indicating the obviously clear existence of the dynamic interaction between the class I protein and the antigen peptide. The interaction of the protein and peptide was discussed as well as the surrounding atmosphere of the peptide in the complex.

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