Related Experiment Video

Updated: Jul 25, 2026

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
06:43

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Published on: May 26, 2021

ClC-2 contributes to native chloride secretion by a human intestinal cell line, Caco-2

R Mohammad-Panah1, K Gyomorey, J Rommens

  • 1Programme in Cell Biology and Genetics at the Hospital for Sick Children and the Departments of Physiology and Molecular Genetics at the University of Toronto, Toronto, M5G 1X8 Ontario, Canada.

The Journal of Biological Chemistry
|November 30, 2000
PubMed
Summary

ClC-2 chloride channels contribute to intestinal chloride secretion. This finding provides a potential therapeutic target for diseases like cystic fibrosis, where chloride transport is impaired.

More Related Videos

A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines06:59

A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

Published on: June 22, 2022

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting10:08

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

Related Concept Videos

Transcellular Transport of Solutes01:23

Transcellular Transport of Solutes

Transcellular transport of solutes is the movement of substances like monosaccharides and amino acids through polarized cells. This transport mechanism is primarily seen in epithelial and endothelial cells aided by membrane transport proteins such as channels and transporters. The tight junctions between these cells confine the membrane proteins to the two sides of the cell. The epithelial cells have distinct apical and basolateral domains. In contrast, the endothelial cells show the luminal...
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...
Transport Number01:31

Transport Number

The transport number is the fraction of the total current carried by an ion in an electrolyte solution. It is defined as the ratio of the current carried by a specific ion to the total current flowing through the solution. The transport number, t, is central to understanding ionic mobility, which describes how fast an ion moves under the influence of an electric field. This link connects the physical behavior of ions in solution to the chemical processes that occur during electrochemical...
Reabsorption and Secretion in the Loop of Henle01:17

Reabsorption and Secretion in the Loop of Henle

The thick ascending limb of the nephron loop has Na+–K+–2Cl− symporters in the apical membranes of its cells. These symporters simultaneously reclaim one sodium ion, one potassium ion, and two chloride ions from the tubular fluid. Sodium ions are actively transported into the interstitial fluid at the base and sides of the cell, diffusing into the vasa recta. Chloride ions move through leakage channels in the basolateral membrane into the interstitial fluid and then into the vasa recta.
Reabsorption and Secretion in the DCT and Collecting Duct01:26

Reabsorption and Secretion in the DCT and Collecting Duct

The early phase of the DCT manages the reabsorption of approximately 10-15% of filtered water, 5–10% of filtered sodium, and 5–10% of filtered chloride. This process is facilitated by Na+–Cl− symporters in apical membranes and sodium-potassium pumps, as well as Cl− leakage channels in basolateral membranes. The early DCT also stands out as a site where parathyroid hormone (PTH) stimulates calcium reabsorption, depending on the body's requirements.
The distal part of the DCT, along with the...
Roles of Electrolytes: Chloride and Bicarbonate01:29

Roles of Electrolytes: Chloride and Bicarbonate

Chloride ions contribute to the osmotic pressure gradient distinguishing the intracellular fluid (ICF) from the extracellular fluid (ECF). They counterbalance positively charged ions in the ECF and ensure its electrochemical stability. The renal system's process of chloride absorption and release generally mirrors that of sodium ions.
Conditions such as hypochloremia can arise from insufficient chloride reabsorption by the kidneys, often compounded by extended bouts of diarrhea, vomiting, or...