Related Experiment Videos
Oligonucleotide labeling: synthesis of a new spin-labeled 2'-deoxyguanosine analogue
C Giordano1, F Pedone, P Fattibene
1Centro di Chimica del Farmaco, CNR, Dipartimento di Studi Farmaceutici, Università La Sapienza, Rome, Italy.
Nucleosides, Nucleotides & Nucleic Acids
|November 30, 2000
Abstract:
In order to achieve an EPR sensitive probe for DNA, 3-carboxy-Proxyl free radical was linked to O-6 of dG through a five-atoms-tether. The modified base was incorporated into a 30-mer ODN, then annealed to its complementary DNA strand. Hydrodynamic parameters show only a slight destabilization with respect to the equivalent unlabeled hybrid. EPR could monitor the hybrid formation showing a progressive enlargement of the upfield signal in passing from the labeled ss- to the ds-30-mer.