Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human calcium transport protein CaT1.

J B Peng1, X Z Chen, U V Berger

  • 1Membrane Biology Program, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.

Biochemical and Biophysical Research Communications
|December 1, 2000
PubMed
Summary

Researchers identified the human calcium transport protein (hCaT1), crucial for intestinal calcium absorption. This protein is found in various tissues, suggesting a broad role in cellular calcium uptake and transport.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Genome-wide association and epidemiological analyses reveal common genetic origins between uterine leiomyomata and endometriosis.

Nature communications·2022
Same author

AnFiSA: An open-source computational platform for the analysis of sequencing data for rare genetic disease.

Journal of biomedical informatics·2022
Same author

Genome-wide association and epidemiological analyses reveal common genetic origins between uterine leiomyomata and endometriosis.

Nature communications·2019
Same author

Target-enriched massively parallel sequencing for genetic diagnosis of hereditary hearing loss in patients with normal array CGH result.

Hong Kong medical journal = Xianggang yi xue za zhi·2018
Same author

Prenatal diagnosis of chromothripsis, with nine breaks characterized by karyotyping, FISH, microarray and whole-genome sequencing.

Prenatal diagnosis·2014
Same author

Disruption of MBD5 contributes to a spectrum of psychopathology and neurodevelopmental abnormalities.

Molecular psychiatry·2013

Area of Science:

  • Molecular Biology
  • Physiology
  • Genetics

Background:

  • Transcellular calcium transport is vital in epithelial tissues like the intestine, kidney, and placenta.
  • A rat calcium transport protein (CaT1) involved in intestinal calcium uptake was previously identified.
  • Understanding human calcium transport mechanisms is essential for physiological and pathological studies.

Purpose of the Study:

  • To identify and characterize the human ortholog of the rat CaT1 protein.
  • To investigate the tissue distribution and gene localization of the human calcium transport protein.
  • To determine the functional properties of the human calcium transport protein in calcium uptake.

Main Methods:

  • Identification of the human ortholog (hCaT1) of rat CaT1.

Related Experiment Videos

  • Analysis of hCaT1 messenger RNA (mRNA) expression in various human tissues and cell lines using transcript analysis.
  • Gene assignment to chromosome 7 using fluorescence in situ hybridization (FISH).
  • Functional characterization of hCaT1 by expressing it in Xenopus laevis oocytes and measuring calcium (Ca2+) uptake.

    • Main Results:

    • The human ortholog, hCaT1, was identified and its mRNA was found in the gastrointestinal tract, pancreas, placenta, prostate, salivary gland, liver, kidney, and testis.
    • hCaT1 mRNA was also detected in colorectal cancer (SW480) and leukemia (K-562) cell lines.
    • The hCaT1 gene was localized to chromosome 7q33-34.
    • Expressed hCaT1 in oocytes demonstrated saturable Ca2+ uptake with a Michaelis constant (Km) of 0.25 mM.

    Conclusions:

    • hCaT1 plays a significant role in cellular calcium uptake across various human tissues.
    • The identified hCaT1 protein is likely involved in the transcellular calcium transport pathway, particularly in the intestine.
    • Further research into hCaT1 function may provide insights into calcium homeostasis and related disorders.