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Transcriptional activation analysis using bioluminescent reporter assays

D R Hodge1, P A Clausen

  • 1Laboratory for Molecular Immunoregulation, NCI-FCRDC-NIH, Frederick, MD, USA. hodge@ncifcrf.gov

Molecular Biotechnology
|December 1, 2000
PubMed

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...