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[Differentiation of yeasts in mastitis milk]
B Keller1, P Scheibl, E Bleckmann
1Institut für Mikrobiologie und Tierseuchen, Tierärztliche Hochschule Hannover, Germany.
Abstract:
Differentiating 174 yeasts from clinical samples to get a survey of those species found in mastitis milk and milking machines we identified five genera with 29 species. Species of the genus Candida dominated with 77% of all samples. More than 25% of yeasts from milk were identified as C. rugosa and more than 20% as C. catenulata. The six species found most often represented 74% of all yeasts in this group. Yeasts from milking machines were more heterogeneous.
Insights
This study surveyed yeasts in mastitis milk and milking machines, identifying 29 species. Candida species predominated, with C. rugosa and C. catenulata being the most common in milk samples.
Area of Science:
- Microbiology
- Veterinary Science
- Food Safety
Background:
- Yeasts are opportunistic pathogens that can cause mastitis in dairy animals.
- Contamination of milk and milking equipment with yeasts poses a risk to milk quality and animal health.
- Understanding yeast species prevalence is crucial for effective control strategies.
Purpose of the Study:
- To identify and quantify yeast species present in clinical mastitis milk samples and on milking machines.
- To determine the dominant yeast genera and species in these environments.
- To compare yeast diversity between milk and milking machine samples.
Main Methods:
- Isolation and identification of yeasts from 174 clinical samples (milk and milking machines).
- Species-level identification using standard microbiological techniques.
- Quantitative analysis of yeast prevalence and distribution.
Main Results:
- A total of 29 yeast species from five genera were identified.
- The genus Candida accounted for 77% of all isolated yeasts.
- Candida rugosa ( >25%) and Candida catenulata ( >20%) were the most prevalent species in milk.
- Six species comprised 74% of all yeasts found in milk.
- Yeasts from milking machines exhibited greater species heterogeneity compared to milk samples.
Conclusions:
- Candida species, particularly C. rugosa and C. catenulata, are significant yeast contaminants in mastitis milk.
- The findings highlight the importance of monitoring and controlling yeast populations in dairy environments.
- Further research into the sources and transmission routes of these yeasts is warranted.
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