Preparation of sperm-free seminal plasma from human semen

Whole human semen was centrifuged at various centrifugal forces to obtain sperm-free seminal plasma. Most samples of seminal plasma collected after centrifguation of up to 10,000 g were contaminated with spermatozoa. Centrifugation at 40,000 g yielded sperm-free seminal plasma in two thirds of the samples centrifuged. Filtration of whole semen with Millipore filters (pore size 0.45 mu) removed all spermatozoa.