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Donor neutrophil function after plateletpheresis.

K H Western¹, V Videm

¹Department of Immunology, Institute of Laboratory Medicine, the Regional Hospital, Trondheim, Norway.

|December 2, 2000

Summary

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Apheresis systems slightly alter neutrophil function, with increased L-selectin expression and reduced oxygen radical production. However, complement activation was not observed, indicating apheresis is a biotolerant procedure for donors.

Area of Science:

Immunology
Hematology

Background:

Neutrophils are key inflammatory mediators.
Apheresis systems may activate neutrophils via foreign surfaces.
Investigating donor neutrophil function post-apheresis is crucial as most white blood cells (WBCs) are returned.

Purpose of the Study:

To assess alterations in donor neutrophil function after apheresis.
To compare the effects of three different apheresis systems (Amicus, Autopheresis-C, CS-3000) on neutrophil function.

Main Methods:

Blood samples from 10 donors were collected pre- and post-apheresis using three systems in random order.
Neutrophil phagocytosis, oxidative burst, L-selectin, and CD11b expression were measured via flow cytometry.
Plasma myeloperoxidase, lactoferrin, C3bc, and terminal complement complex levels were quantified.

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Main Results:

Neutrophil L-selectin expression increased (p=0.02), while oxygen radical production decreased (p=0.01) post-apheresis, suggesting potential priming.
No significant differences in neutrophil function were observed among the three apheresis systems.
Complement activation was not detected.

Conclusions:

Apheresis causes minor alterations in neutrophil function.
The interaction between neutrophils and apheresis system surfaces is biotolerant.
Donor neutrophil function remains largely unaffected by standard apheresis procedures.