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Capillary zone electrophoresis in phosphate buffer--known or unknown?
P Gebauer1, P Pantůiková, P Bocek
1Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno.
Journal of Chromatography. A
|December 2, 2000
Summary
Phosphate buffers, commonly used in capillary electrophoresis, can exhibit multiple centers of symmetry. This finding explains extreme peak dispersion observed in electropherograms, impacting analytical accuracy.
Area of Science:
- Analytical Chemistry
- Physical Chemistry
- Electrochemical Methods
Background:
- Capillary electrophoresis (CE) analysis can be affected by regions of extreme peak dispersion.
- This dispersion is often linked to the presence of multiple centers of symmetry within the electrolyte system.
- Understanding these phenomena is crucial for accurate electrophoretic separations.
Purpose of the Study:
- To investigate the presence of multiple centers of symmetry in phosphate buffer systems.
- To determine if phosphate buffers contribute to extreme electromigration dispersion in CE.
- To validate findings through experimental evidence.
Main Methods:
- Utilized the peak shape diagram approach to analyze electrolyte systems.
- Examined neutral and alkaline phosphate buffer solutions.
- Conducted model experiments to confirm theoretical observations.
Main Results:
- Revealed that neutral and alkaline phosphate buffers possess two centers of symmetry.
- Identified one center of extreme dispersion within these buffer systems.
- Experimental data corroborated the existence of multiple symmetry centers and extreme dispersion.
Conclusions:
- Phosphate buffers, widely used in CE, can exhibit complex symmetry properties.
- The presence of multiple centers of symmetry in phosphate buffers directly leads to extreme peak dispersion.
- This discovery necessitates a re-evaluation of CE methods employing phosphate buffers for enhanced analytical precision.