Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Construction of large-volume monolithic columns.

A Podgornik1, M Barut, A Strancar

  • 1BIA Separations d.o.o., Ljubljana, Slovenia.

Analytical Chemistry
|January 11, 2000
PubMed
Summary

Researchers developed a mathematical model to create large-volume monolithic supports for chromatography. This enables the production of uniform, 80 mL monolithic units with stable performance at high flow rates.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Report of 16SrIII-B Phytoplasma Subgroup Associated with Virescence of Arnica montana in Serbia.

Plant disease·2019
Same author

First Report of Group 16SrXII-A Phytoplasma Causing Stolbur Disease in Saponaria officinalis Plants in Serbia.

Plant disease·2019
Same author

First Report of Xanthomonas campestris pv. campestris as the Causal Agent of Black Rot on Oilseed Rape (Brassica napus) in Serbia.

Plant disease·2019
Same author

First Report of Blueberry Reddening Disease in Serbia Associated with 16SrXII-A (Stolbur) Phytoplasma.

Plant disease·2019
Same author

First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia.

Plant disease·2019
Same author

First Report of 16SrXII-A Subgroup Phytoplasma (Stolbur) Associated with Reddening of Oenothera biennis in Serbia.

Plant disease·2019

Area of Science:

  • Chromatography
  • Materials Science
  • Chemical Engineering

Background:

  • Monolithic supports offer advantages for chromatography but scaling up production is challenging.
  • Exothermic polymerization in GMA-EDMA monoliths causes temperature increases, limiting uniform structure formation in larger volumes.

Purpose of the Study:

  • To analyze heat release during polymerization and develop a model for predicting the maximum thickness of uniform monolithic annuli.
  • To successfully prepare and assemble large-volume monolithic units (80 mL) for chromatographic applications.

Main Methods:

  • Mathematical modeling of heat release during polymerization to predict structural uniformity limits.
  • Polymerization of GMA-EDMA to form monolithic annuli and their subsequent merging into a larger unit.

Related Experiment Videos

  • Design of a specialized housing for uniform radial flow distribution within the monolithic column.

    • Main Results:

    • A mathematical model was derived to predict the maximal thickness of monolithic annuli with uniform structure.
    • Two monolithic annuli were polymerized and merged into an 80 mL monolithic unit.
    • The monolithic column demonstrated flow-independent separation efficiency and dynamic binding capacity up to flow rates exceeding 100 mL/min.
    • Low pressure drop (≤ 2.5 MPa at 250 mL/min) and rapid separation/loading times (minutes) were achieved.

    Conclusions:

    • The developed mathematical model facilitates the creation of larger, uniform monolithic supports.
    • The 80 mL monolithic column shows excellent performance characteristics, including flow independence and high capacity, suitable for large-scale chromatographic separations.
    • This work overcomes previous limitations in scaling up monolithic column technology.