[Determination of serum digitoxin by means of a solid-phase enzyme-linked immunosorbent assay (author's transl)]

A solid-phase ELISA-technique for clinical applications is described. It requires little apparatus other than a photometer and allows the rapid determination of serum digitoxin levels. It differs from the original technique only in the sample volume being smaller and a digitoxin calibration curve being required. Concurrent assays by a reference method (86Rb-erythrocyte assay) gave results in excellent agreement with the values obtained by the modified ELISA-technique on samples from patients under long-term therapy with digitoxin (r = 0.95).