Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Randomly amplified polymorphic DNA analysis of Erysipelothrix spp.

A T Okatani1, H Hayashidani, T Takahashi

  • 1Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.

Journal of Clinical Microbiology
|December 2, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fatal Salmonellosis in Captive Maras (Dolichotis patagonum) Caused by Salmonella Enteritidis.

Journal of comparative pathology·2017
Same author

Aberrant forms of Yersinia pseudotuberculosis as spheroplasts and filaments in yersiniosis in squirrel monkeys.

Veterinary pathology·2014
Same author

Herd prevalence of Enterobacteriaceae producing CTX-M-type and CMY-2 β-lactamases among Japanese dairy farms.

Journal of applied microbiology·2013
Same author

Outbreak of yersiniosis in Egyptian rousette bats (Rousettus aegyptiacus) caused by Yersinia pseudotuberculosis serotype 4b.

Journal of comparative pathology·2012
Same author

Prevalence of Salmonella, Yersinia and Campylobacter spp. in feral raccoons (Procyon lotor) and masked palm civets (Paguma larvata) in Japan.

Zoonoses and public health·2011
Same author

Pathological changes in captive monkeys with spontaneous yersiniosis due to infection by Yersinia enterocolitica serovar O8.

Journal of comparative pathology·2010

The randomly amplified polymorphic DNA (RAPD) method effectively identifies Erysipelothrix species and aids in epidemiological analysis. Specific primers NK51 and NK6 offer rapid and reliable differentiation of strains within this genus.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Accurate identification of Erysipelothrix species is crucial for disease control and epidemiological studies.
  • Existing methods may lack the specificity or speed required for rapid differentiation.
  • The genus Erysipelothrix includes important animal and human pathogens.

Purpose of the Study:

  • To evaluate the utility of the randomly amplified polymorphic DNA (RAPD) method for species identification within the genus Erysipelothrix.
  • To assess the potential of RAPD for epidemiological analysis of Erysipelothrix strains.
  • To identify specific random primers effective for differentiating Erysipelothrix species and strains.

Main Methods:

  • Testing of 81 Erysipelothrix strains against 18 random primers using the RAPD technique.

Related Experiment Videos

  • Analysis of DNA band patterns generated by primers NK51 and NK6.
  • Comparison of RAPD profiles with known species and serovar classifications.

    • Main Results:

    • Primer NK51 generated species-specific patterns, differentiating E. rhusiopathiae and E. tonsillarum, and identified potential misclassifications.
    • Primer NK6 revealed genetic variations among strains, enabling differentiation within the same serovar.
    • The RAPD method demonstrated high discriminatory power for Erysipelothrix strains.

    Conclusions:

    • The RAPD method, particularly with primers NK51 and NK6, is a rapid, reliable, and cost-effective tool for Erysipelothrix species identification.
    • This technique is valuable for epidemiological surveillance and strain differentiation within the genus Erysipelothrix.
    • RAPD analysis can aid in correcting taxonomic misclassifications and understanding pathogen diversity.