Evaluation of random amplified polymorphic DNA typing of Pseudomonas aeruginosa
M Campbell1, E Mahenthiralingam, D P Speert
1Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, and Children's and Women's Health Centre of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada.
Journal of Clinical Microbiology
|December 2, 2000
Abstract:
A method for distinguishing among Pseudomonas aeruginosa strains using random amplified polymorphic DNA (RAPD) typing was evaluated for reproducibility and discriminatory power. A total of 200 isolates, blinded in triplicate, were evaluated by RAPD. All 600 samples were typeable; 197 of 200 isolates gave identical results on all three occasions, and 131 distinct RAPD types were identified.


