Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Slow phase hemolysis in hypotonic electrolyte solutions.

T K Chan, P L LaCelle, R I Weed

    Journal of Cellular Physiology
    |February 1, 1975
    PubMed
    Summary

    Erythrocyte hemolysis occurs in fast and slow phases. Slow phase hemolysis requires critical swelling and membrane stress, not just osmotic pressure, leading to irreversible changes and reduced deformability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Time Pattern of Presentation of Victims of High-Speed Passenger Ferry Mass Casualty Incidents to the Emergency Department.

    Disaster medicine and public health preparedness·2024
    Same author

    Gas kinematics in FIRE simulated galaxies compared to spatially unresolved HI observations.

    Monthly notices of the Royal Astronomical Society·2019
    Same author

    The origin of ultra diffuse galaxies: stellar feedback and quenching.

    Monthly notices of the Royal Astronomical Society·2018
    Same author

    Clinical outcome of HIV-infected haemophiliac patients in Hong Kong.

    Haemophilia : the official journal of the World Federation of Hemophilia·2016
    Same author

    Synthesis of embedded Au nanostructures by ion irradiation: influence of ion induced viscous flow and sputtering.

    Beilstein journal of nanotechnology·2014
    Same author

    Low-dose subcutaneous alemtuzumab is a safe and effective treatment for chronic acquired pure red cell aplasia.

    Hong Kong medical journal = Xianggang yi xue za zhi·2013

    Area of Science:

    • Cell Biology
    • Biophysics
    • Hematology

    Background:

    • Erythrocyte hemolysis exhibits distinct fast and slow phases.
    • Understanding the mechanisms of slow phase hemolysis is crucial for cell membrane research.

    Purpose of the Study:

    • To investigate the factors contributing to the slow phase of erythrocyte hemolysis.
    • To elucidate the role of membrane stress and water removal in hypotonic lysis.

    Main Methods:

    • Partial hemolysis of erythrocytes using hypotonic media.
    • Analysis of osmotic fragility and cell deformability.
    • Assessment of sucrose protection during hemolysis.

    Main Results:

    • Slow phase hemolysis is dependent on critical non-hemolytic swelling and membrane strain.
    • Sucrose protection involves counter-balancing osmotic pressure and removing intracellular water.
    • Hypotonic stress induces irreversible membrane changes, including stomatocytosis and wrinkling.
    • Cells undergoing slow phase hemolysis show reduced deformability.

    Conclusions:

    • Slow phase hemolysis is a complex process involving membrane integrity beyond simple osmotic pressure.
    • Hypotonic stress can cause permanent alterations in erythrocyte membrane structure and function.
    • These findings contribute to models of cell volume regulation and membrane mechanics.

    Related Experiment Videos