Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phage display in pharmaceutical biotechnology.

S S Sidhu1

  • 1Department of Protein Engineering, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. sidhu@gene.com

Current Opinion in Biotechnology
|December 5, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multicenter double-blind, placebo-controlled randomized trial to evaluate the safety and efficacy of bovine colostrum in the treatment of severe alcoholic hepatitis (SAH).

Trials·2023
Same author

Author Correction: High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression.

Nature communications·2022
Same author

High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression.

Nature communications·2022
Same author

Sorafenib induced hepatic encephalopathy.

Acta gastro-enterologica Belgica·2018
Same author

A panel of synthetic antibodies that selectively recognize and antagonize members of the interferon alpha family.

Protein engineering, design & selection : PEDS·2017
Same author

Intratumoral heterogeneity: pathways to treatment resistance and relapse in human glioblastoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2017

Recent advancements in M13 phage display technology have enhanced library construction and introduced new formats. These improvements facilitate the isolation of specific protein ligands and the study of binding interactions, expanding the applications of phage display in biological research.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biochemistry

Background:

  • M13 phage display is a powerful technique for discovering protein-protein interactions and identifying ligands.
  • Continuous innovation in library construction and display formats is crucial for expanding its utility.

Purpose of the Study:

  • To summarize recent improvements in M13 phage display methods.
  • To highlight the expanded applications of phage display technologies.

Main Methods:

  • Development of improved M13 phage-display library construction techniques.
  • Introduction of novel phage display formats.
  • Application of phage display for ligand isolation and binding interaction studies.

Main Results:

Related Experiment Videos

  • Significant methodological advancements in M13 phage display library construction.
  • Development of new display formats enhancing the technology's versatility.
  • Successful isolation of specific ligands for protein targets using phage-displayed peptide libraries.
  • Utility of phage antibody and cDNA libraries demonstrated for defining binding interactions.
  • Phage display methods now enable rapid determination of binding energetics at protein-protein interfaces.

Conclusions:

  • Recent innovations have significantly enhanced M13 phage display capabilities.
  • The technology's applications have broadened, impacting ligand discovery and interaction analysis.
  • Phage display remains a vital tool for molecular interaction studies and drug discovery.