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Current methodologies for the analysis of aminoglycosides
Journal of Chromatography. B, Biomedical Sciences and Applications
|December 5, 2000
Summary
Aminoglycoside antibiotics, crucial in medicine and agriculture, are analyzed using diverse methods. This review details quantitative techniques like HPLC and immunoassays for accurate drug determination and residue detection.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Biochemistry
Background:
- Aminoglycosides are a diverse antibiotic class with essential roles in clinical, veterinary, and agricultural applications.
- Their structure features aminosugars linked to an aminocyclitol component, with over 30 key compounds identified.
Purpose of the Study:
- To comprehensively review qualitative and quantitative analytical methods for aminoglycosides.
- To evaluate the suitability of various techniques for different applications, from bulk pharmaceutical analysis to residue detection and therapeutic drug monitoring.
Main Methods:
- Qualitative methods discussed include X-ray crystallography, NMR spectroscopy, and MS.
- Quantitative methods encompass microbiological assays, immunoassays (RIA, EIA, FIA), spectrophotometry, chromatography (GC, TLC, HPLC), and capillary electrophoresis (CE).
Main Results:
- Spectrophotometric methods suffice for bulk pharmaceuticals.
- Microbiological assays are useful for semi-quantitative screening of food residues.
- Enzyme immunoassays offer accuracy for complex matrices, while automated immunoassays are optimal for therapeutic drug monitoring.
- HPLC provides necessary specificity and sensitivity for research, and HPLC-MS confirms veterinary drug residues.
Conclusions:
- The choice of aminoglycoside analysis method depends on the specific application, matrix, and required accuracy.
- HPLC and CE show potential for further development in analyzing biological samples.
- Accurate determination of aminoglycosides is vital across various fields, necessitating appropriate analytical strategies.