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Related Experiment Videos

Induced lactation in prepubertal Holstein heifers.

S Ball1, K Polson, J Emeny

  • 1PPL Therapeutics Inc., Virginia Tech Corporate Research Center, Blacksburg 24061, USA.

Journal of Dairy Science
|December 5, 2000
PubMed
Summary

Hormonal induction of lactation in prepubertal heifers successfully produced milk secretions for biochemical testing. This method did not affect reproduction or subsequent natural lactation, proving useful for transgenic research.

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Area of Science:

  • Animal Science
  • Biotechnology
  • Endocrinology

Background:

  • Hormonal induction of lactation in prepubertal animals is a potential method for producing biological compounds.
  • Transgenic livestock offer a platform for producing therapeutic recombinant proteins.
  • Early detection of gene expression in transgenic cattle is crucial for research and development.

Purpose of the Study:

  • To assess the feasibility of hormonally inducing lactation in prepubertal Holstein heifers.
  • To evaluate the yield and composition of milk obtained from induced heifers.
  • To determine the impact of induced lactation on subsequent natural lactation and reproductive performance.

Main Methods:

  • Prepubertal Holstein heifers (approx. 6 months old) received daily injections of estrogen and progesterone, followed by dexamethasone.

Related Experiment Videos

  • Twice-daily hand milking commenced post-hormonal treatment.
  • Milk yield, composition (lactose, protein, fat), and serum alpha-lactalbumin levels were analyzed.

    • Main Results:

    • Lactation was successfully induced in 5 out of 6 heifers, yielding 32-500 ml of milk by day 7.
    • Milk composition averaged 4.7% lactose, 4.1% protein, and 3.7% fat.
    • Induced heifers showed increased serum alpha-lactalbumin post-dexamethasone treatment; no adverse effects on reproduction or first natural lactation were observed.

    Conclusions:

    • Hormonal induction of lactation in prepubertal heifers is a viable technique for obtaining sufficient secretions for biochemical testing.
    • This method is a valuable tool for evaluating mammary-linked gene constructs in transgenic cattle.
    • While not practical for dairy production, it supports advancements in biotechnology and therapeutic protein development.