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Related Experiment Videos

Widespread recombinase expression using FLPeR (flipper) mice.

F W Farley1, P Soriano, L S Steffen

  • 1Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

Genesis (New York, N.Y. : 2000)
|December 6, 2000
PubMed
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Researchers created a new mouse strain, FLPeR, for enhanced gene recombination. This FLPeR mouse model facilitates genetic studies across most tissues, including the germ line, supporting reproducible research.

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Advancing conditional genetic strategies necessitates diverse site-specific recombinase systems.
  • Existing tools may not fully address the need for broad-spectrum gene manipulation.
  • The ROSA26 locus is a well-established site for constitutive gene expression in mice.

Purpose of the Study:

  • To develop a novel mouse model for generalized expression of the enhanced FLP recombinase (FLPe).
  • To establish a resource for extensive target gene recombination across multiple tissue types.
  • To provide a tool for generating FLPe-expressing cell lines and serving as a deleter strain.

Main Methods:

  • Targeting the constitutive ROSA26 locus to achieve generalized FLPe expression.

Related Experiment Videos

  • Generation and characterization of the FLPeR mouse strain.
  • Assessment of recombination efficiency in various tissue types, including germ line cells.

    • Main Results:

    • Successful creation of the FLPeR mouse strain with constitutive FLPe expression.
    • Demonstration of extensive target gene recombination in most tissue types, including the developing germ line.
    • FLPeR mice serve as a valuable source for FLPe-expressing primary cell lines and as a deleter strain.

    Conclusions:

    • The FLPeR mouse strain provides a powerful tool for conditional genetic studies.
    • Its utility spans primary cell line generation, gene deletion strategies, and germ line manipulation.
    • Preservation of a 129 genetic background in crossed progeny aids in maintaining reproducible results and genetically defined controls.