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Expression and cellular localization of contraception-associated protein
A Wagenfeld1, C H Yeung, S Shivaji
1Institute of Reproductive Medicine, University of Münster, Germany.
Journal of Andrology
|December 6, 2000
Summary
Ornidazole treatment causes reversible infertility in male rats by affecting contraception-associated protein 1 (CAP1) on sperm. This protein
Area of Science:
- Reproductive Biology
- Molecular Biology
- Biochemistry
Background:
- Ornidazole induces reversible infertility in male rats.
- Contraception-associated protein 1 (CAP1) is detected in epididymal fluid after ornidazole treatment.
- CAP1 shares similarity with DJ-1, a human oncogene.
Purpose of the Study:
- To investigate the role of CAP1 in male rat fertility and its changes during ornidazole-induced infertility.
- To characterize CAP1 gene expression and protein localization in rat testes and sperm.
Main Methods:
- Gene cloning and characterization.
- Reverse transcription of RNA from various species.
- In situ hybridization for cellular RNA expression.
- Immunolocalization for protein localization.
- Flow cytometry for sperm surface protein analysis.
Main Results:
- CAP1 gene is expressed in testes of multiple species, including humans.
- CAP1 shows stage-specific expression in spermatocytes and spermatids in rat testes.
- A translational delay in CAP1 expression was observed in elongating spermatids.
- Ornidazole treatment reduced sperm surface CAP1 levels and altered its location.
- CAP1 is found on the sperm head's equatorial segment and cytoplasmic droplets.
Conclusions:
- CAP1 plays a potential role in the fertilization process.
- Changes in CAP1 expression and localization are associated with ornidazole-induced infertility.
- CAP1 is a conserved protein with potential implications in reproductive health.