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Updated: Oct 10, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
04:11

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA

Published on: December 15, 2023

High-speed detection of blood-borne hepatitis C virus RNA by single-tube real-time fluorescence reverse

D Ratge1, B Scheiblhuber, M Nitsche

  • 1Department of Clinical Chemistry and Laboratory Medicine, Robert Bosch Hospital, Auerbachstrasse 110, D-70376 Stuttgart, Germany. Dieter.Ratge@RBK.de

Clinical Chemistry
|December 6, 2000
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...