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Updated: Aug 11, 2026

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
10:51

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

A PKC-eta/Fyn-dependent pathway leading to keratinocyte growth arrest and differentiation

S Cabodi1, E Calautti, C Talora

  • 1Cutaneous Biology Research Center Harvard Medical School and Massachusetts General Hospital, Charlestown, MA 02129, USA.

Molecular Cell
|December 7, 2000
PubMed

Insights

Increased Fyn kinase activity suppresses epithelial cell growth by downregulating EGF receptor signaling. Protein kinase C-eta directly activates Fyn, controlling keratinocyte growth and differentiation.

Area of Science:

  • Cell biology
  • Biochemistry
  • Dermatology

Background:

  • Epithelial cell growth regulation differs significantly from other cell types.
  • Fyn, a Src kinase family member, is crucial for keratinocyte differentiation.
  • Protein kinase C-eta (PKC-eta) is also involved in keratinocyte growth and differentiation control.

Purpose of the Study:

  • To investigate the role of Fyn kinase and PKC-eta in regulating epithelial cell growth and differentiation.
  • To elucidate the signaling pathways involved in keratinocyte growth suppression.

Main Methods:

  • Investigated the effect of Fyn kinase activation on keratinocyte and dermal fibroblast growth.
  • Analyzed the impact of Fyn activity on epidermal growth factor receptor (EGFR) signaling.
  • Examined the relationship between PKC-eta activity and Fyn activation in keratinocytes.

Main Results:

  • Increased Fyn activity suppressed keratinocyte growth, but not dermal fibroblasts, via EGFR signaling downmodulation.
  • PKC-eta activity was both necessary and sufficient for Fyn activation.
  • PKC-eta and Fyn were found to associate, with recombinant PKC-eta directly activating Fyn.

Conclusions:

  • A direct crosstalk exists between PKC-eta and Fyn in keratinocytes.
  • This PKC-eta and Fyn signaling axis governs the balance between epithelial cell growth and differentiation.

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