Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydrogen peroxide augments eosinophil adhesion via beta2 integrin.

M Nagata1, H Yamamoto, M Shibasaki

  • 1Pulmonary Division, Second Department of Internal Medicine, Saitama Medical School, Saitama, Japan.

Immunology
|December 7, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isolation and identification of C-type natriuretic peptide in human monocytic cell line, THP-1.

Biochemical and biophysical research communications·1992
Same author

Stable expression of natriuretic peptide receptors: effects of HS-142-1, a non-peptide ANP antagonist.

Biochemical and biophysical research communications·1992
Same author

Gestational age-dependent dual action of epidermal growth factor on human placenta early in gestation.

The Journal of clinical endocrinology and metabolism·1992
Same author

[The prevalence and the clinical characteristics of silent myocardial ischemia detected by stress thallium scintigraphy].

Kaku igaku. The Japanese journal of nuclear medicine·1992
Same author

Inherited heterozygous protein C deficiency and dysfunctional protein S with recurrent venous thrombotic diseases: a study of three generations of a Japanese family.

Internal medicine (Tokyo, Japan)·1992
Same author

Cloning and characterization of an androgen-induced growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells.

Proceedings of the National Academy of Sciences of the United States of America·1992

Hydrogen peroxide (H2O2) enhances eosinophil (EOS) adhesion to endothelial cells by increasing beta2 integrin expression. This finding sheds light on eosinophil

Area of Science:

  • Immunology
  • Cell Biology
  • Allergic Inflammation Research

Background:

  • Eosinophil (EOS) accumulation is crucial in allergic inflammation, involving EOS binding to endothelial cells (EC).
  • Previous studies showed EOS adhesion to vascular cell adhesion molecule-1 (VCAM-1) stimulates EOS respiratory burst.
  • Reactive oxygen species, like hydrogen peroxide (H2O2), may alter EOS adhesive properties.

Purpose of the Study:

  • To investigate whether H2O2 directly modifies the adhesive properties of eosinophils.
  • To determine the specific adhesion molecules and integrins involved in H2O2-mediated EOS adhesion.

Main Methods:

  • Isolated peripheral blood eosinophils from healthy donors.
  • Assessed EOS adhesion to tumor necrosis factor-alpha (TNF-α) stimulated or resting human umbilical vein endothelial cells (HUVEC) with or without H2O2.

Related Experiment Videos

  • Utilized specific antibodies (anti-β2 integrin, anti-CD11b, anti-CD11a, anti-α4 integrin) to block adhesion.
  • Examined EOS adhesion to recombinant ICAM-1 and VCAM-1.
  • Measured CD11b and CD18 expression on EOS.

    • Main Results:

    • H2O2 significantly enhanced EOS adhesion to both resting and TNF-α-stimulated HUVEC.
    • This enhancement was inhibited by anti-β2 integrin and anti-CD11b antibodies, but not by anti-CD11a or anti-α4 integrin antibodies.
    • H2O2 increased EOS adhesion to ICAM-1 but not VCAM-1.
    • H2O2 upregulated the expression of CD11b and CD18 on EOS.

    Conclusions:

    • Hydrogen peroxide directly augments eosinophil adhesive properties.
    • The mechanism involves the upregulation of β2 integrin (CD11b/CD18) on eosinophils.
    • This finding provides insight into eosinophil recruitment in inflammatory conditions.