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Updated: Oct 10, 2026
Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
Published on: September 16, 2013
Laser-capture microdissected schwannian and neuroblastic cells in stage 4 neuroblastomas have the same genetic
1Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Background:
Neuroblastoma (NB) is a heterogeneous tumor composed of mixed populations of neuroblastic, Schwannian, and gangliocytic cells.
Procedure:
We examined the genetic lineage of Schwannian and neuroblastic cells in stage 4 NB using laser-capture microdissection (LCM) and allelic analysis of microsatellite markers of chromosomes 1p, 11q, and 14q.
Results:
In 18 out of 19 cases, individual cell types exhibited the same allelotype.
Conclusion:
Our results provide evidence that the majority of Schwannian and neuroblastic cells in stage 4 NB are of neoplastic origin, and support the hypothesis of a tumor stem cell capable of development along multiple lineages.
