Related Experiment Video
Updated: Oct 10, 2026
FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
Chromosome 2 short arm translocations revealed by M-FISH analysis of neuroblastoma cell lines
N Van Roy1, H Van Limbergen, J Vandesompele
1Center of Medical Genetics, Ghent University Hospital, Gent, Belgium.
Procedure:
M-FISH analysis was performed on 18 neuroblastoma cell lines, which were previously studied with cytogenetic, standard FISH and CGH data.
Results:
One of the most striking findings of this study was the detection of chromosome 2 short arm rearrangements in 61% of the investigated cell lines. These rearrangements resulted from translocations with various partner chromosomes. All translocations, except one were unbalanced, leading to the consistent gain of chromosome segment 2pter-p22. A cryptic balanced translocation t(2;4) was observed with a breakpoint located in the vicinity of MYCN in cell line NBL-S.
Conclusions:
Combination of M-FISH results together with cytogenetic, standard FISH and CGH data yielded the most comprehensive description of chromosome 2 short arm rearrangements, leading to a consistent gain of chromosome 2 short arm material.
