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Updated: Aug 5, 2026
High Content Screening in Neurodegenerative Diseases
Published on: January 6, 2012
Hunting the subset-specific genes of neuroblastoma: expression profiling and differential screening of the
M Ohira1, T Shishikura, T Kawamoto
1Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba, Japan.
Background:
Neuroblastoma (NBL) has a distinct nature in different prognostic subgroups.
Procedure:
To understand the molecular mechanism of NBL's genesis and biology as well as that of the neural crest development, we constructed full-length-enriched cDNA libraries by an oligo-capping method from two different subsets of primary NBL, one with favorable biology and the other with MYCN amplification.
Results:
Sequencing analysis of these libraries revealed that the expression profile was markedly different between both subsets. To identify the genes differentially expressed between the subsets, semi-quantitative RT-PCR analyses are proceeding.
Conclusion:
So far, 54 transcripts have been found to be expressed at high levels in favorable NBLs, and significantly at low levels in unfavorable NBLs.
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