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Tyrosine hydroxylase-based DNA-vaccination is effective against murine neuroblastoma
1The Scripps Research Institute, Department of Immunology, La Jolla, California, USA. holger.lode@charite.de
Background:
The disruption of self-tolerance against neuroblastoma is the ultimate goal of an effective DNA-vaccine.
Procedure:
Here we demonstrate the induction of a protective immunity against syngeneic murine NXS2 neuroblastoma in A/J mice, following vaccination with tyrosine hydroxylase (TH) derived antigens. Oral gene delivery was accomplished using an attenuated strain of Salmonella typhimurium as a carrier harboring vectors encoding for mTH antigens.
Results:
Vaccination was effective in protecting animals from a lethal challenge with wild-type NXS2 tumor cells.
Conclusions:
These results provide the first evidence of the TH self antigen being recognized by T-cells and demonstrate that a TH-based DNA vaccine is a potentially useful immunotherapeutic strategy for neuroblastoma.