Differential expression in an antisense MYCN neuroblastoma model
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Updated: Oct 10, 2026
Zebrafish Model of Neuroblastoma Metastasis
Published on: March 14, 2021
M L Schmidt1, A Lal, D Dittmann
1Department of Pediatrics, University of Illinois at Chicago, Chicago, Illinois, USA. mls3@uic.edu
Background:
A previously published antisense MYCN-expressing model system was utilized to identify genes whose expression is altered by the down-regulation of MYCN by AS MYCN.
Results:
Differential display comparing nontransfected human NB NBL-S cells to three AS MYCN-expressing cell lines (NBAS-4, -5, and -6) yielded nine differentially expressed cDNAs designated NDDE:1-9, for MYCN-dependent differential expression genes. A GenBank search revealed matches for seven of the nine cDNAs. Differential expression was confirmed for five of the cDNAs by Northern blot analysis.
Results:
NDDE:8 is up-regulated in the AS MYCN-expressing clones and shares homology with the EB1 clone p53-induced gene (PRG3). NDDE:2 is up-regulated in the high-expressing N-myc protein NBL-S cells and shares homology with a 27 kD heat shock protein PAN1 clone. Further analysis of all five cDNAs might further elucidate the targets of MYCN in NB.
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