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[Tridimensional skin models recording percutaneous absorption]

Gysler1, Königsmann, Schäfer-Korting

  • 1Frei Universit&aumlt; Berlin, Institut für Pharmazie, Abteilung Pharmakologie und Toxikologie, D-Berlin.

ALTEX
|December 7, 2000
PubMed
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This study compares reconstructed human epidermis models for percutaneous absorption testing. Skinethictrade mark demonstrated superior barrier function and reproducibility compared to EpiDermtrade mark and Episkintrade mark, showing promise as an animal experiment alternative.

Area of Science:

  • Dermatology
  • Toxicology
  • In vitro testing

Background:

  • Lack of validated alternatives to animal testing for percutaneous absorption.
  • Need for reliable reconstructed human epidermis models to assess skin penetration.

Purpose of the Study:

  • Compare barrier function of three reconstructed epidermis models (Episkintrade mark, EpiDermtrade mark, Skinethictrade mark) against human skin.
  • Evaluate batch-to-batch variation in these models.
  • Assess potential as alternatives for percutaneous absorption studies.

Main Methods:

  • Transepidermal water loss (TEWL) measurements.
  • Prednisolone (PD) penetration assays.
  • Comparison with excised human skin.
  • Batch-to-batch variation analysis.

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Main Results:

  • Skinethictrade mark exhibited superior barrier function (lower PD penetration, 150 ng/ml) compared to EpiDermtrade mark (300 ng/ml).
  • Episkintrade mark showed the poorest barrier function (500 ng/ml PD penetration).
  • Skinethictrade mark demonstrated better batch-to-batch reproducibility than human skin.
  • Human skin had the lowest TEWL (9 g·m-2·h-1) and undetectable PD levels.

Conclusions:

  • Skinethictrade mark shows significant promise as a reliable in vitro model for percutaneous absorption evaluation.
  • Its superior barrier function and reproducibility make it a viable alternative to animal testing.
  • Further research into human skin equivalents for percutaneous absorption is warranted.