Related Experiment Videos
[Infectivity and resistance to antibiotics of uropathogenic gram-negative rods]
Z Drulis-Kawa1, E Lewczyk, S Jankowski
1Laboratorium Bakteriologiczne Wojewódzkiego Specjalistycznego Szpitala Chorób Dzieciecych im. J. Korczaka we Wrocławiu.
Insights
Escherichia coli is the primary cause of pediatric urinary tract infections (UTIs). While many E. coli strains show resistance to ampicillin, they remain sensitive to beta-lactamase inhibitor combinations, indicating effective treatment options for childhood UTIs.
Area of Science:
- Pediatric infectious diseases
- Microbiology
- Antimicrobial resistance
Background:
- Urinary tract infections (UTIs) are common in children.
- Identifying causative bacterial agents and their antibiotic susceptibility is crucial for effective treatment.
- Antibiotic resistance patterns are evolving, necessitating regular surveillance.
Purpose of the Study:
- To characterize bacterial isolates from children with suspected UTIs.
- To determine the antibiotic susceptibility profiles of these urinary pathogens.
- To inform clinical management strategies for pediatric UTIs.
Main Methods:
- Bacterial isolates were collected from pediatric patients with clinically suspected UTIs.
- Isolates were identified using standard microbiological techniques.
- Antibiotic susceptibility testing was performed on significant isolates against selected antibiotics.
Main Results:
- Escherichia coli was the predominant pathogen, accounting for 67% of isolates.
- Proteus mirabilis was the second most common isolate (10%).
- Over 50% of E. coli isolates exhibited resistance to amino-penicillins, but >80% were sensitive to beta-lactamase inhibitor combinations.
- A significant proportion (32.5%) of Klebsiella pneumoniae isolates were Extended-Spectrum Beta-Lactamase (ESBL) producers.
Conclusions:
- E. coli is the leading cause of UTIs in the studied pediatric population.
- Resistance to common antibiotics like amino-penicillins is prevalent in E. coli.
- Combinations of beta-lactamase inhibitors with beta-lactams remain effective against most E. coli isolates.
- The high rate of ESBL production in K. pneumoniae highlights the need for careful antibiotic selection.
Abstract:
The nature of bacterial isolates from children with clinically suspected urinary tract infections (UTI) was studied. The susceptibility of urinary pathogens to selected antibiotics was determined. The results clearly show that E. coli was identified as the main causative agent of UTI children (67% of isolates). The second commonest pathogen was P. mirabilis (10%). Over half E. coli isolates were resistant to amino-penicilins but almost all isolates (over 80%) were sensitive to antimicrobial agents combined with beta-lactamase inhibitors. We found significantly high percentage (32.5%) of ESBL strains among K. pneumoniae isolates.