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Polymerase chain reaction for definitive identification of Yersinia ruckeri
1Department of Veterinary Microbiology and Pathology and the Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman 99164, USA.
Abstract:
Yersinia ruckeri causes enteric red mouth (ERM) disease in salmonids. Serologic identification of Y. ruckeri is hampered by cross-reactivity with other bacterial isolates of fish origin. Oligonucleotide primers incorporating Y. ruckeri unique sequences were designed to amplify a 409 bp fragment of Y. ruckeri 16S rDNA. The primers did not amplify other genetically related Yersinia or a wide variety of other aquatic or piscine bacteria. This assay provides a rapid, definitive identification of Y. ruckeri that is not subject to the variability inherent in serologic methods.