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Related Experiment Videos

The BRG-1 subunit of the SWI/SNF complex regulates CD44 expression.

M W Strobeck1, M F DeCristofaro, F Banine

  • 1Department of Cell Biology, University of Cincinnati College of Medicine, Vontz Center for Molecular Studies, Cincinnati, Ohio 45267-0521, USA.

The Journal of Biological Chemistry
|December 18, 2000
PubMed
Summary

Aberrant CD44 regulation impacts tumor growth. Loss of BRG-1 protein deficiency causes CD44 down-regulation, affecting cellular adhesion and metastasis.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Aberrant CD44 regulation is implicated in tumor growth and metastasis.
  • The mechanism of CD44 down-regulation in tumors remains unclear.
  • CD44 plays a role in cellular adhesion and metastasis.

Purpose of the Study:

  • Investigate the mechanism of CD44 down-regulation in cervical carcinoma cells.
  • Identify the role of BRG-1 in CD44 expression and regulation.
  • Explore the link between BRG-1, Cyclin E, and CD44 in tumor progression.

Main Methods:

  • Immunoblot and reverse transcription-polymerase chain reaction (RT-PCR) analysis.
  • Cell fusion experiments between CD44-deficient (C33A) and CD44-expressing (SAOS-2) cell lines.

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  • Reintroduction of BRG-1 and use of dominant-negative BRG-1 to assess its function.

    • Main Results:

    • Cervical carcinoma cell line C33A lacks CD44 expression.
    • Cell fusion restored CD44 expression, indicating a trans-acting factor.
    • BRG-1 deficiency correlated with CD44 absence in tumor cell lines.
    • Reintroducing BRG-1 restored CD44 expression.
    • Cyclin E overexpression attenuated CD44 stimulation, consistent with BRG-1 abrogation.

    Conclusions:

    • BRG-1 is a critical regulator of CD44 expression.
    • Loss of BRG-1 function may be a general mechanism for CD44 down-regulation in tumors.
    • SWI/SNF components, through BRG-1, are implicated in regulating cellular adhesion and metastasis via CD44.