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Detection and elimination of contaminating microorganisms in transplantable tumors and cell lines
1Developmental Research Laboratory, Nippon Shinyaku Co., Ltd., Kyoto, Japan.
Abstract:
As a quarantine of biological materials, we tested 96 transplantable tumors and cell lines for contamination with microorganisms in a mouse antibody production (MAP) test, enzymatic assay and microbiological culture. Contamination with lactic dehydrogenase elevating virus (LDV), mycoplasmas and Pasteurella pneumotropica was detected. A considerable difference in the contamination rate was observed between in vivo- and in vitro- propagated tumors. LDV in the tumors could be eliminated by both in vitro subculture and subpassage in nude rats. Mycoplasmas were eliminated by means of the mycoplasma-removal agent and P. pneumotropica by subpassage in mice. These results suggest that there is still a high risk of contamination in transplantable tumors and emphasizes the importance of adequate microbiological quality control.
Insights
Microbiological contamination, including lactic dehydrogenase elevating virus (LDV), mycoplasmas, and Pasteurella pneumotropica, is a significant risk in transplantable tumors. Effective elimination strategies exist, highlighting the need for stringent quality control in biological material quarantine.
Area of Science:
- Microbiology
- Cell Biology
- Animal Models
Background:
- Transplantable tumors and cell lines are crucial research tools.
- Microbiological contamination poses a significant threat to experimental validity.
- Existing quarantine protocols require rigorous microbiological assessment.
Purpose of the Study:
- To assess the microbial contamination status of 96 transplantable tumors and cell lines.
- To identify common contaminants and compare contamination rates between in vivo and in vitro propagated tumors.
- To evaluate the efficacy of different methods for eliminating detected contaminants.
Main Methods:
- Mouse antibody production (MAP) test.
- Enzymatic assays.
- Microbiological cultures.
- In vitro subculture and subpassage in nude rats or mice.
- Mycoplasma removal agents.
Main Results:
- Contamination with lactic dehydrogenase elevating virus (LDV), mycoplasmas, and Pasteurella pneumotropica was detected in tested samples.
- A notable difference in contamination rates was observed between tumors propagated in vivo versus in vitro.
- LDV was eliminated by in vitro subculture and subpassage in nude rats.
- Mycoplasmas were eradicated using a mycoplasma-removal agent.
- Pasteurella pneumotropica was eliminated through subpassage in mice.
Conclusions:
- Transplantable tumors carry a substantial risk of microbial contamination.
- Adequate microbiological quality control is essential for biological material quarantine.
- Specific methods can effectively eliminate common contaminants like LDV, mycoplasmas, and P. pneumotropica.