Acetylcholine-induced relaxation in rat ocular vasculature

Endothelial cell function is often evaluated by the assessment ofendothelium-dependent relaxation to acetylcholine. The purpose of this study was to characterize the acetylcholine-induced vasodilator responses in the intact ocular vasculature tree in isolated perfused rat eyes. At a fixed perfusion flow rate (5 microl/min), the resultant perfusion pressure reflected the total vascular resistance of the perfused vasculature. The baseline vascular resistance, and hence perfusion pressure, was manipulated by perfusion with high concentrations of potassium Krebs (60, 90, or 124 mM). The response to bolus administration of acetylcholine (10(-8) to 10(-4) M) was then assessed over a range of perfusion pressures. Acetylcholine induced a dose-dependent relaxation response which was larger in magnitude with increased levels of induced vascular tone. The relationship between the size of the acetylcholine-induced response and the baseline perfusion pressure was essentially linear with a correlation coefficient of 0.86 at 10(-5) M and of 0.88 at 10(-4) M acetylcholine. Our results suggest that, in the isolated perfused rat eye, the initial vascular tone has to be considered when evaluating endothelial cell function by acetylcholine-induced relaxation.