[The application of competitive RT-PCR to detect minimal residual disease in patients with 8;21 translocation]
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Published on: March 29, 2017
1Jiangsu Institute of Haematology, the First Affiliated Hospital of Suzhou Medical College, Suzhou, Jiangsu, 215006 P.R.China.
Objective:
To construct and evaluate the competitive quantitative RT-PCR method for detecting the fusion gene of AML1-ETO in the patients of t(8;21)AML(acute myeloid leukemia).
Methods:
The authors used the method of splicing by overlapping extension (SOE) to obtain the competitive DNA fragment, with which they set up the competitive quantitative RT-PCR assay to detect AML1-ETO chimeric genes in the patients of t(8;21) AML.
Results:
The competitive DNA fragment has been obtained; the sensitive competitive RT-PCR method is developed, which allows the quantitation of the number of AML1-ETO transcriptions in t(8;21)AML patients at different phases.
Conclusion:
The competitive quantitative RT-PCR method, based on SOE, is simple and convenient; t(8;21) AML patients in different survival conditions express AML1-ETO gene quite differently.
