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Related Concept Videos

Transformation01:26

Transformation

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Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
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Genetic transformation systems in insects.

P W Atkinson1, A C Pinkerton, D A O'Brochta

  • 1Department of Entomology, University of California, Riverside, California 92521, USA. peter.atkinson@ucr.edu

Annual Review of Entomology
|December 9, 2000
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Summary
This summary is machine-generated.

New genetic tools enable insect transformation, aiding gene function studies. However, current methods require further development for robust application and risk assessment is crucial for genetically engineered insects.

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Area of Science:

  • Entomology
  • Genetics
  • Molecular Biology

Background:

  • Recent advancements have enabled stable genetic transformation in non-drosophilid insects.
  • Transposable-element-based and virus-based techniques offer new genetic tools for insect research.

Purpose of the Study:

  • To review and compare emerging gene transfer systems for insects.
  • To assess the current state and future requirements of insect genetic tools.
  • To discuss risk assessment for genetically engineered insects.

Main Methods:

  • Comparative analysis of transposable-element-based and virus-based gene transfer systems.
  • Literature review of recent developments in insect genetic transformation techniques.
  • Examination of risk assessment considerations for genetically engineered insects.

Main Results:

  • Several novel genetic tools for insect transformation have emerged in the last five years.
  • These systems provide initial capabilities for studying gene function and regulation in insects.
  • Current genetic tools are not yet considered robust enough for widespread, reliable application.

Conclusions:

  • Significant progress has been made in insect genetic transformation technologies.
  • Further development is needed to meet the requirements of robust genetic tools for insects.
  • Risk assessment is a critical consideration for the responsible development and release of genetically engineered insects.