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Updated: May 26, 2026

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Published on: December 3, 2013

Recombinant bovine adenovirus type 3 expressing bovine viral diarrhea virus glycoprotein E2 induces an immune

M K Baxi¹, D Deregt, J Robertson

¹Virology Group, Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan, S7N 5E3, Canada.

|December 9, 2000

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Recombinant bovine adenovirus 3 (BAV-3) vectors were engineered to express bovine viral diarrhea virus (BVDV) glycoprotein E2. Intranasal immunization induced specific immune responses, demonstrating BAV-3

Area of Science:

Virology and Immunology
Gene Therapy and Vaccine Development

Background:

Recombinant bovine adenovirus (BAV) is explored for animal vaccination and human gene therapy.
Bovine viral diarrhea virus (BVDV) glycoprotein E2 is a target antigen for immune response induction.

Purpose of the Study:

To construct replication-competent BAV-3 recombinants expressing BVDV E2 glycoprotein.
To evaluate the efficacy of these recombinants as mucosal vaccines.

Main Methods:

Two BAV-3 recombinants (BAV331, BAV338) were created expressing BVDV E2 in the BAV-3 E3 region.
E2 gene expression utilized either a modified BAV-3 promoter or a CMV promoter.
Bovine herpesvirus 1 (BHV-1) glycoprotein D signal sequence was fused to E2 for proper processing.

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Last Updated: May 26, 2026

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Published on: December 3, 2013

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Main Results:

Recombinant E2 protein (53 kDa, dimerizing to 94 kDa) was successfully expressed and recognized by antibodies.
Replication of BAV-3 was not affected by the insertion of the E2-expression cassette.
Intranasal immunization of cotton rats elicited E2-specific IgA and IgG responses in mucosa and serum.

Conclusions:

Bovine adenovirus 3 can efficiently express pestivirus glycoprotein E2.
Replication-competent BAV-3 vectors are effective for inducing specific mucosal and systemic immune responses against the expressed antigen.