Co-replication of a reovirus and a polydnavirus in the ichneumonid parasitoid Hyposoter exiguae

A recently established colony of the ichneumonid parasitoid, Hyposoter exiguae, was found to carry both a reovirus (HeRV) and a polydnavirus (HePDV). Morphogenesis of these viruses was observed in all cells comprising the ovarian calyx epithelium, apparently without detrimental effect to the parasitoid. While polydnavirus replication in H. exiguae was restricted to the calyx region, HeRV was detected in ovarioles, oviducts, midguts, malpighian tubules, and accessory glands associated with the male reproductive system. In addition, HeRV was able to infect the fat body of parasitized host larvae and to establish a persistent infection in vitro. Electron microscopy revealed that both viruses were released into the calyx fluid compartment exclusively by budding, a phenomenon rarely observed among the Reoviridae; HeRV envelopes thus obtained, however, appeared to be subsequently shed within the oviducts. HeRV particles were concentrated to near homogeneity by differential centrifugation; mature virions consisted of seven to eight structural polypeptides and 10 dsRNA genome segments. Prominent spikes were observed at the vertices of icosahedral core particles. Most, but not all, individuals comprising the H. exiguae colony appeared to be infected with HeRV, suggesting a commensal relationship between wasp and virus; however, while this association is of obvious benefit to the virus, it seems unlikely that any advantage accrues to the parasitoid which carries it.