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Updated: May 5, 2026

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
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Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

Published on: October 9, 2013

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Expression of c-erbB receptors and ligands in human nasal epithelium

R Polosa1, G Prosperini, V Tomaselli

  • 1Division of Respiratory, Cell & Molecular Biology, Faculty of Medicine, University of Southampton, Southampton General Hospital, Southampton, United Kingdom.

The Journal of Allergy and Clinical Immunology
|December 12, 2000
PubMed
Abstract

Insights

Epidermal Growth Factor (EGF) receptors and ligands are present in human nasal epithelium. This coexpression provides a foundation for studying EGF receptor-mediated effects in nasal tissues.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Otorhinolaryngology

Background:

  • The epidermal growth factor (EGF) family is crucial for epithelial tissue maintenance and repair.
  • Limited information exists on the coexpression of EGF family members and their c-erbB receptors in human nasal epithelium.

Purpose of the Study:

  • To investigate the expression of EGF family ligands and c-erbB receptors in cultured human nasal epithelial cells and nasal mucosa.
  • To establish a basis for understanding c-erbB receptor-mediated effects in the nasal epithelium.

Main Methods:

  • Reverse transcription PCR (RT-PCR) to detect mRNA.
  • Western blotting to confirm protein expression.
  • Immunohistochemistry to localize receptors and ligands in tissue.

Main Results:

  • mRNA for EGF receptors (EGFR) c-erbB2 and c-erbB3, but not c-erbB4, was detected in cultured nasal epithelial cells.
  • Transcripts for EGF, heparin-binding EGF, transforming growth factor (TGF) alpha, and amphiregulin were also found.
  • Immunohistochemistry confirmed EGFR, c-erbB2, and c-erbB3 expression in nasal mucosa, co-localizing with their ligands.

Conclusions:

  • Coexpression of c-erbB receptors and their ligands is demonstrated in human nasal epithelium.
  • This finding supports further investigation into c-erbB receptor-mediated signaling pathways in nasal tissues.

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