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Updated: Aug 18, 2026
A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
Published on: March 5, 2018
Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis
1Department of Bioengineering, Box 357962, University of Washington, Seattle, WA 98195-7962, USA. narendra@u.washington.edu
Abstract:
This report describes a part of the evolution of microgel electrophoresis in the author's laboratory for the last 15 years. It also describes the importance of estimation of DNA single and double strand breaks, DNA crosslinks and apoptosis. Some experiments based on each methodology are included here. A new protocol for rapid and efficient precipitation of DNA in microgel is included. A step by step description of laboratory protocol is also included.
