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Determination of D1 and D2 dopamine receptor expression by Ntera-2 cells
1Department of Pharmacology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140, USA.
Abstract:
There is evidence that D1 and D2 dopamine receptors are co-expressed on some neurons. As a potential model of co-expression we examined Ntera-2 cells using RT-PCR, and showed that they express D2 but not D1 receptors. D2 dopamine receptor expression was confirmed by quinpirole inhibition of forskolin-stimulated cAMP formation. Absence of D1 dopamine receptors was confirmed by the inability of dopamine or SKF 81297 to increase cAMP.
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