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Related Experiment Videos

In vivo cell electrofusion.

H Mekid1, L M Mir

  • 1Laboratoire de Physicochimie et Pharmacologie des Macromolécules Biologiques, UMR 8532 CNRS, Institut Gustave-Roussy, 39 rue C. Desmoulins, F-94805 Cédex, Villejuif, France.

Biochimica Et Biophysica Acta
|December 13, 2000
PubMed
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Researchers report the first instance of cell fusion occurring within living tissues using electric pulses. This in vivo electrofusion, observed in melanoma tumors, shows potential for gene therapy and cancer treatment.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Oncology

Background:

  • In vitro electrofusion is a well-established technique for fusing cells using electric pulses.
  • In vivo applications of cell electropermeabilization are expanding rapidly.
  • The occurrence and implications of in vivo cell electrofusion remain largely unexplored.

Purpose of the Study:

  • To report the novel occurrence of cell fusion within living tissues (in vivo) induced by electric pulses.
  • To investigate the parameters influencing in vivo cell electrofusion, including electric field strength and tissue type.
  • To describe the morphological and kinetic changes associated with in vivo cell electrofusion and its potential impact on biomedical applications.

Main Methods:

  • Utilized the B16 melanoma tumor model in vivo.

Related Experiment Videos

  • Applied permeabilizing electric pulses to induce cell electropermeabilization (electroporation).
  • Monitored morphological changes, syncytia/giant cell formation, mitosis, and cell death kinetics.
  • Examined tissue-specific dependence of electrofusion by testing liver and other tumor types.

    • Main Results:

    • Successfully demonstrated in vivo cell electrofusion within metastasizing B16 melanoma tumors.
    • Electrofusion efficacy correlated with the applied voltage-to-electrode distance ratio, indicating dependence on in vivo electroporation.
    • Observed syncytial areas and giant cells, with specific kinetics for mitosis and cell death.
    • Electrofusion was not observed in liver or other tested tumor types, suggesting tissue-specific requirements like reduced extracellular matrix.

    Conclusions:

    • In vivo cell electrofusion is achievable in specific tumor microenvironments, such as the B16 melanoma model.
    • Tissue-specific conditions, potentially involving extracellular matrix composition, are critical for successful in vivo electrofusion.
    • In vivo cell electrofusion may offer advantages for gene therapy (DNA electrotransfer) and antitumor electrochemotherapy, particularly for metastasizing tumors.