Related Experiment Videos
Isolation of 5 alpha- and 5 beta-dihydrorubrosterone from Silene otites L. (Wib)
M Báthori1, J P Girault, I Máthé
1Department of Pharmacognosy, Albert Szent-Györgyi Medical University, H-6701 Szeged, Eötvös u. 6, P.O. Box 121, Hungary. bathori@pharma.szote.u-szeged.hu
Biomedical Chromatography : BMC
|December 13, 2000
Abstract:
5 alpha-Dihydrorubrosterone (2 beta, 3 beta, 14 alpha, 17 beta-tetrahydroxy-5 alpha-androst-7-ene-6-one), a new 19-carbon 5 alpha-ecdysteroid, was isolated together with its 5 beta counterpart from the aerial parts of Silene otites L. (Wib.) (Caryophyllaceae) by a combination of solvent partition, low-pressure column chromatography, thin-layer chromatography (normal-phase and reversed-phase) and finally HPLC. Mass spectrometry and nuclear magnetic resonance spectroscopic procedures were used for compound characterization.