Application of capillary electrophoresis to the analysis of soluble chromatin

N V Asatiani¹, M K Abuladze, B G Birkaya

¹Institute of Physics, Georgian Academy of Sciences, Tamarashvili str. 6, Tbilisi, Georgia.

|December 13, 2000

Abstract:

Capillary electrophoresis (CE) has been applied to study DNA-protein complexes using as the test system soluble chromatin from chicken erythrocytes and rapidly proliferated cultured Chinese hamster fibroblast-like cells B11-dii-FAF-28. Separation was performed with home-made CE apparatus, using a regulated high-voltage power supply, UV-detector and fused silica capillaries with inner diameter 75 microm. The heterogeneity of nucleosomal particles with different DNA lengths after micrococcal nuclease digestion was detected.

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