Associative memory hamiltonians for structure prediction without homology: alpha-helical proteins

Energy landscape theory is used to obtain optimized energy functions for predicting protein structure, without using homology information. At short sequence separation the energy functions are associative memory Hamiltonians constructed from a database of folding patterns in nonhomologous proteins and at large separations they have the form of simple pair potentials. The lowest energy minima provide reasonably accurate tertiary structures even though no homologous proteins are included in the construction of the Hamiltonian. We also quantify the funnel-like nature of these energy functions by using free energy profiles obtained by the multiple histogram method.