Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

On the dynamics of TCR:CD3 complex cell surface expression and downmodulation.

H Liu1, M Rhodes, D L Wiest

  • 1Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38101, USA.

Immunity
|December 15, 2000
PubMed
Summary

T-cell receptor (TCR) downmodulation occurs via intracellular retention and degradation, not increased internalization, following antigen binding. This process, driven by constitutive internalization, facilitates serial T cell activation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

JUN is a key transcriptional regulator of the unfolded protein response in acute myeloid leukemia.

Leukemia·2016
Same author

Transcription factors NFAT2 and Egr1 cooperatively regulate the maturation of T-lymphoma in vitro.

Biochemistry. Biokhimiia·2007
Same author

Branching out to gain control: how the pre-TCR is linked to multiple functions.

Immunology today·2000
Same author

Competitive displacement of pT alpha by TCR-alpha during TCR assembly prevents surface coexpression of pre-TCR and alpha beta TCR.

Journal of immunology (Baltimore, Md. : 1950)·2000
Same author

Identification of a novel pre-TCR isoform in which the accessibility of the TCR beta subunit is determined by occupancy of the 'missing' V domain of pre-T alpha.

International immunology·2000
Same author

Multiplexed particle-based flow cytometric assays.

Journal of immunological methods·2000

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • T-cell receptor (TCR) downmodulation after MHC:peptide complex ligation is crucial for T cell activation.
  • Understanding the dynamics of TCR:CD3 complex expression is key to deciphering T cell signaling.

Purpose of the Study:

  • To investigate the dynamics of TCR:CD3 cell surface expression in resting and activated T cells.
  • To elucidate the mechanisms underlying TCR downmodulation upon antigen-specific ligation.

Main Methods:

  • Analysis of TCR:CD3 complex cell surface expression dynamics.
  • Investigation of internalization and recycling rates post-TCR ligation.
  • Assessment of degradation pathways (lysosomes and proteasomes).

Related Experiment Videos

Main Results:

  • The TCR:CD3 complex exhibits high stability with rapid internalization and recycling in resting T cells.
  • TCR ligation does not increase the rate of internalization, despite causing downmodulation.
  • TCR downmodulation results from intracellular retention and subsequent degradation of ligated complexes.

Conclusions:

  • TCR downmodulation is primarily mediated by preventing the recycling of ligated complexes, not by inducing increased internalization.
  • Constitutive internalization, rather than ligation-induced changes, drives serial TCR ligation events.
  • These findings offer new insights into T cell activation regulation and immune response dynamics.