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Related Experiment Videos

Temperature control in a continuously mixed bioreactor for solid-state fermentation.

F J Nagel1, J Tramper, M S Bakker

  • 1Wageningen University, Department of Food Technology and Nutritional Sciences, Food and Bioprocess Engineering Group; P.O. Box 8129, 6700 EV, Wageningen, The Netherlands.

Biotechnology and Bioengineering
|December 15, 2000
PubMed
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Continuous mixing in solid-state fermentation (SSF) with Aspergillus oryzae improved temperature control and substrate homogeneity. This method shows promise for large-scale SSF, though evaporative cooling remains crucial.

Area of Science:

  • Biotechnology
  • Biochemical Engineering

Background:

  • Solid-state fermentation (SSF) is crucial for producing various biomaterials.
  • Scaling up SSF presents challenges in controlling temperature and moisture content.
  • Previous SSF systems often lacked efficient mixing, leading to inhomogeneities.

Purpose of the Study:

  • To evaluate the effectiveness of a continuously mixed, aseptic paddle mixer for large-scale SSF.
  • To assess the impact of continuous mixing on temperature control, homogeneity, and fungal viability.
  • To investigate the role of evaporative cooling and water addition in mixed SSF systems.

Main Methods:

  • Utilized a continuously mixed, aseptic paddle mixer for SSF with Aspergillus oryzae on whole wheat kernels.
  • Monitored respiration rates to assess fungal health and substrate integrity.

Related Experiment Videos

  • Performed scale-up calculations to determine cooling requirements.
  • Validated an enthalpy balance model for process control under evaporative cooling.

    • Main Results:

    • Continuous mixing successfully controlled temperature and prevented bed inhomogeneities.
    • Respiration rates were comparable to unmixed systems, indicating minimal fungal damage.
    • Continuous mixing enhanced heat transport, reducing reliance on evaporative cooling.
    • Scale-up analysis revealed the continued necessity of evaporative cooling for large volumes.
    • Homogeneous water addition and process control were demonstrated using an enthalpy balance.

    Conclusions:

    • Continuous mixing offers significant advantages for temperature and moisture control in large-scale SSF.
    • Evaporative cooling remains essential for large-scale SSF, requiring careful water management.
    • The validated enthalpy balance provides a pathway for automated process control in mixed SSF systems.