Related Experiment Video
Updated: Aug 4, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Characterization of a putative pathogenicity island from bovine Staphylococcus aureus encoding multiple superantigens
J R Fitzgerald1, S R Monday, T J Foster
1Department of Microbiology, Moyne Institute of Preventive Medicine, Republic of Ireland. rfitzgerald@niaid.nih.gov
A novel bovine pathogenicity island (SaPIbov) in Staphylococcus aureus encodes toxins like toxic shock syndrome toxin (TSST) and staphylococcal enterotoxin C (SEC). This island influences the bovine immune response by stimulating specific T cells.
Area of Science:
- Bacteriology
- Immunology
- Molecular Genetics
Background:
- Staphylococcus aureus causes bovine mastitis.
- Some strains produce toxic shock syndrome toxin (TSST) and staphylococcal enterotoxin C (SEC).
Purpose of the Study:
- To characterize a novel pathogenicity island (SaPIbov) in Staphylococcus aureus strain RF122.
- To investigate the role of SaPIbov in modulating the bovine immune response.
Main Methods:
- Molecular genetic analysis of SaPIbov.
- Construction of TSST and SEC-deficient mutants using allele replacement.
- In vitro bovine lymphocyte expansion assays.
Main Results:
- SaPIbov encodes TSST (tst), bovine SEC (sec-bovine), and an enterotoxin-like gene (sel).
- SaPIbov is integrated adjacent to the GMP synthase gene and shares sequence identity with SaPI1.
- TSST stimulates BTB13-specific T cells, while SEC-bovine stimulates BTB93-specific T cells.
Conclusions:
- SaPIbov is a mobile genetic element potentially acquired recently.
- The toxins encoded by SaPIbov modulate the bovine immune response by activating specific T cell populations.
More Related Videos
09:26Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Related Concept Videos
Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Determinants of Bacterial Pathogenicity and Virulence
Regulation of Bacterial Virulence
Staphylococcal Skin Infections
Bacterial Gastroenteritis
Clinical Significance of Antibiotic Resistance