Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks
1Dept of Biomolecular Sciences, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, M60 1QD, Manchester, UK. finbarr.hayes@bi.umist.ac.uk
Abstract:
Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity.
More Related Videos
06:50Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions
Published on: January 26, 2024
05:56Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches
Published on: October 13, 2022
Related Concept Videos
Leaky Scanning
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Bacterial Protein Maturation
Mutations
